Inhibition of human platelet aggregation by choisyaternatine isolated from Choisya ternata (Rutaceae)

Ikarastika Rahayu Abdul Wahab, and Nikki Siu Hai Wong, and Maria Jose Santoz-Martinez, and Fabio Boylan, (2015) Inhibition of human platelet aggregation by choisyaternatine isolated from Choisya ternata (Rutaceae). In: International Conference on Natural Products 2015, 24-25 March 2015, Universiti Teknologi Malaysia, Johor Bahru.


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A new alkaloid was isolated from the hexane extract of Choisya ternata Kunth. (Rutaceae) (Figure 1a) leaves, 6, 8-Dimethoxy-7-(3-methylbut-2-enyloxy) quinolin-2-ol (choisyaternatine) (1), together with the known compounds skimmianine (2), Lup-20(29)-en-3β-ol (lupeol) (3), Lup-20(29)-en-3β,24-diol (4) and tecleamaniensine A (5). Compounds 3-5 are reported for the first time in this species. The structures of isolated compounds were elucidated by extensive spectroscopic analyses and comparison with previously reported spectroscopic data. The structure of choisyaternatine was further confirmed based on X-ray data analysis. Anti-platelet activity of hexane extract, choisyaternatine and skimmianine was also evaluated. The extract was able to significantly inhibit collagen-induced platelet aggregation at all concentrations tested (25, 50 and 100 μg/ml). The new compound, choisyaternatine, has shown a significant inhibition of collagen-induced platelet aggregation demonstrated in a concentration dependent manner from 250 μM (Figure 1b-c). The concentrations which inhibit 50% of platelet aggregation (IC₅₀) were found to be 564 μM for skimmianine and 698 μM for choisyaternatine. This is also the first report for the antiplatelet activity for this plant and these alkaloids using human platelets.

Item Type: Conference or Workshop Item (Paper)
Keywords: Choisya ternata, choisyaternatine, platelet aggregation
Faculty: Faculty of Agro - Based Industry
Deposited By: En. Wahyudi Yusra Zulfin
Date Deposited: 21 May 2017 08:36
Last Modified: 21 May 2017 08:36

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