Isolation of potential biosurfactant producer from soil contaminated with palm oil mill effluent

Farah Fatin Fasehah Ahmad Fazil, (2016) Isolation of potential biosurfactant producer from soil contaminated with palm oil mill effluent. Undergraduate project report, Faculty of Agro - Based Industry. (Submitted)

Abstract

Biosurfactants are amphiphilic compounds produced by various bacteria and fungi which reduce surface and interfacial tension. The unique properties of biosurfactants make them possible to replace or to be added to synthetic surfactants which are mainly used in food, cosmetics and pharmaceutical industries and in environmental applications. The main objective of this study is to isolate and characterize potential biosurfactant producer from soil contaminated with palm oil mill effluent. Soil samples were collected from overflow mixing pool drain (M1) and anaerobic mixing pool drain (A1) of a palm oil mill. Six potential biosurfactant producers were successfully isolated. The isolation was carried out in Mineral Salt Medium (MSM) supplemented with 1% crude palm oil as sole carbon source for bacterial growth. Production of biosurfactant were screened by emulsification assay, drop collapse assay and oil spreading assay. M1-2 showed higher potential of biosurfactant as it showed highest emulsification index which is 57.88% and also scored positive results for other two test. A1-2 showed lowest emulsification index which was 35%. The higher emulsification index might indicates higher activity of biosurfactant.

Item Type: Undergraduate Final Project Report
Faculty: Faculty of Agro - Based Industry
Call Number: SBT 2016 018
Supervisor: Dr. Ainihayati binti Abdul Rahim
Programme: Bioindustrial Technology
Deposited By: En. Wahyudi Yusra Zulfin
Date Deposited: 24 Aug 2016 01:51
Last Modified: 13 Feb 2017 12:13
URI: http://umkeprints.umk.edu.my/id/eprint/5853

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