Articular sartilage regeneration with autologous marrow aspirate and hyaluronic acid: an experimental study in a goat model

Mohd Azam Khan Goriman Khan, and Khay - Yong Saw, and Paisal Hussin, and Seng-Cheong Loke, (2009) Articular sartilage regeneration with autologous marrow aspirate and hyaluronic acid: an experimental study in a goat model. The Journal of Arthroscopic and Related Surgery, 25 (12). pp. 1391-1400. ISSN 1526-3231

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Abstract

Purpose The purpose of the study was to determine whether postoperative intra-articular injections of autologous marrow aspirate (MA) and hyaluronic acid (HA) after subchondral drilling resulted in better cartilage repair as assessed histologically by Gill scoring. Methods In a goat model we created a 4-mm full-thickness articular cartilage defect in the stifle joint (equivalent to 1.6 cm in the human knee) and conducted subchondral drilling. The animals were divided into 3 groups: group A (control), no injections; group B (HA), weekly injection of 1 mL of sodium hyaluronate for 3 weeks; and group C (HA + MA), similar to group B but with 2 mL of autologous MA in addition to HA. MA was obtained by bone marrow aspiration, centrifuged, and divided into aliquots for cryopreservation. Fifteen animals were equally divided between the groups and sacrificed 24 weeks after surgery, when the joint was harvested, examined macroscopically and histologically. Results Of the 15 animals, 2 from group A had died of non–surgery-related complications and 1 from group C was excluded because of a joint infection. In group A the repair constituted mainly scar tissue, whereas in group B there was less scar tissue, with small amounts of proteoglycan and type II collagen at the osteochondral junction. In contrast, repair cartilage from group C animals showed almost complete coverage of the defect with evidence of hyaline cartilage regeneration. Histology assessed by Gill scoring was significantly better in group C with 1-way analysis of variance yielding an F statistic of 10.611 with a P value of .004, which was highly significant. Conclusions Postoperative intra-articular injections of autologous MA in combination with HA after subchondral drilling resulted in better cartilage repair as assessed histologically by Gill scoring in a goat model. Clinical Relevance After arthroscopic subchondral drilling, this novel technique may result in better articular cartilage regeneration.

Item Type: Non-Indexed Article
Uncontrolled Keywords: Articular cartilage - Arthroscopic subchondral drilling - Hyaline cartilage - Hyaluronic acid - Mesenchymal stem cells - Microfracture
Faculty: Faculty of Veterinary Medicine
Depositing User: En. Pahmi Abdullah
Date Deposited: 27 Jun 2012 17:07
Last Modified: 04 Feb 2013 11:12
URI: http://umkeprints.umk.edu.my/id/eprint/569
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