Production and ptimizoation of cellulase enzyme using aspergillus niger USM AI 1 and comparison with trichoderma reesei via solid state fermentation system

Lee Chee Keong, and Darah Ibrahim, and Ibrahim Che Omar, (2011) Production and ptimizoation of cellulase enzyme using aspergillus niger USM AI 1 and comparison with trichoderma reesei via solid state fermentation system. Biotechnology Research International, 2011. 6 pages. ISSN 2090-3146

Official URL: http://www.hindawi.com/journals/btri/2011/658493/

Abstract

Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; ; aeration at 4 L/ fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U/g of Filter paper activity (FPase) was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by A. niger USM AI 1 was about 35.3% higher compared to Trichoderma reesei. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design

Item Type: Non-Indexed Article
Faculty: Faculty of Agro - Based Industry
Deposited By: En. Pahmi Abdullah
Date Deposited: 23 May 2017 06:35
Last Modified: 23 May 2017 06:35
URI: http://umkeprints.umk.edu.my/id/eprint/5536

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