An improved technique for the rapid chemical characterisation of bacterial terpene cyclases

Jeroen S. Dickschat, and Khomaizon A. K. Pahirulzaman, and Patrick Rabe, and Tim A. Klapschinski, (2014) An improved technique for the rapid chemical characterisation of bacterial terpene cyclases. ChemBioChem, 15 (6). pp. 810-814. ISSN 1439-7633

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/cbic.20...

Abstract

A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases.

Item Type: Non-Indexed Article
Keywords: Actinomycetes, biosynthesis;enzymes, GC/MS, heterologous expression, terpene cyclases
Faculty: Faculty of Agro - Based Industry
Deposited By: En. Pahmi Abdullah
Date Deposited: 21 May 2017 06:52
Last Modified: 21 May 2017 06:52
URI: http://umkeprints.umk.edu.my/id/eprint/5257

Actions (login required)

View Item View Item