Effect of nutritional factors on protease production by isolate pjk 2 2 (1) 2

Chiu, Li Mei (2014) Effect of nutritional factors on protease production by isolate pjk 2 2 (1) 2. Undergraduate project report, Faculty Of Agro - Based Industry. (Submitted)

Abstract

Study on the effect of nutritional factors and optimization for maximum protease production was carried out using the isolated bacteria strain PJK 2 2 (1) 2 which collected from Jeli Hot Spring (Kelantan, Malaysia). The bacterium produced protease at maximum rate after 24 hours of incubation at 50°C with agitation speed of 150 rpm and 4% (v/v) starter culture. The best carbon, organic nitrogen and metal ion sources were glucose, tryptone and calcium chloride with protease activity 39.11 U/ml, 39.67 U/ml and 40.78 U/ml respectively. Meanwhile, the addition of inorganic nitrogen source and amino acid reduced the protease production. The combination of glucose, tryptone and calcium chloride produced the maximum protease production was 40.78 U/ml.

Item Type: Undergraduate Final Project Report
Call Number: SBT 14008
Supervisor: Dr Noor Azlina Ibrahim
Programme: Bio - industrial Technology
Deposited By: En. Wahyudi Yusra Zulfin
Date Deposited: 30 Apr 2015 04:33
Last Modified: 13 Feb 2017 08:44
URI: http://umkeprints.umk.edu.my/id/eprint/3731

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