The existence of vibrio sp. in smoked Asian clam, corbicula fluminea, with emphasis its antibiogram

Norlina Saniman, (2013) The existence of vibrio sp. in smoked Asian clam, corbicula fluminea, with emphasis its antibiogram. [Undergraduate Final Year Project Report] (Submitted)

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Abstract

This study described antimicrobial susceptibility patterns of Vibrio sp. isolated from smoked Asian clam (Corbicula fluminea) was determined by considering that C. fluminea are shellfish that widespread in aquatic environments and widely recognized for became a transmission agent of pathogens in human. The study is carried out to determine the pathogenicity and antibiotic sensitivity of bacteria isolated from Corbicula fluminea sample. In the present study, bacteria isolates from the sample of smoked Corbicula fluminea using Thiosulfate Citrate Bile Sucrose agar (TCBS) and identified through bacterial biological test and conventional identification were investigated for their susceptibility to commercial antibiotics. The isolates were tested against 19 antibiotics (Florfenicol (301.1.μg), Sulphamethoxazole (25μg), Tetracycline (3014), Flumequine (30μg), Oleandomycin (15μg), Colistin sulphate (25μg), Spiramycin (1001μg), Ampicillin (10μg), Erythromycin (15μg), Chloramphenicol (30μg), Novobiocin (30μg), Compound Sulphonamides (300μg), Oxolinic acid (2μg), Gentamicin (12μg), Amoxycillin (25μg), Doxycycline (30μg), Nalidixic (30μg), Lincomycin (15μg) and Fosfomycin (50μg) resulting in 64.21% cases of sensitive, while 4.74% and 31.58% cases of intermediary sensitive and resistant respectively. Vibrio sp. is expected present from smoked Corbicula fluminea and can cause illness to human. Based on the present study, it was found that antibiotics that are effective against Vibrio sp. are Florfenicol (30μg), Tetracycline (30μg), Flumequine (30μg), Chloramphenicol (30μg), Gentamicin (120μg), Amoxycillin (25μg) and Doxycycline (30μg). The study suggests that the effective antibiotics may be used as supplement to agar medium for bacteria selective purpose.

Item Type: Undergraduate Final Year Project Report
Faculty: Faculty of Agro - Based Industry
Depositing User: En. Wahyudi Yusra Zulfin
Date Deposited: 07 Mar 2015 07:23
Last Modified: 16 Feb 2017 01:35
URI: http://umkeprints.umk.edu.my/id/eprint/3507
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