Phylogenetic analysis of different breeds of domestic chickens in selected area of Peninsular Malaysia inferred from partial cytochrome b gene information and RAPD markers

Jayaraj V.K, and Fook Choy Yap, and Yap Jin Yan, and Kiung Teh Loon, and Justina Lee Ning Zhen, and Nelly Warau Kamau, (2010) Phylogenetic analysis of different breeds of domestic chickens in selected area of Peninsular Malaysia inferred from partial cytochrome b gene information and RAPD markers. [Indexed Article]

Official URL: http://www.tandfonline.com/doi/abs/10.1080/1049539...

Abstract

The present investigation was carried out in an attempt to study the phylogenetic analysis of different breeds of domestic chickens in Peninsular Malaysia inferred from partial cytochrome b gene information and random amplified polymorphic DNA (RAPD) markers. Phylogenetic analysis using both neighbor-joining (NJ) and maximum parsimony (MP) methods produced three clusters that encompassed Type-I village chickens, the red jungle fowl subspecies and the Japanese Chunky broilers. The phylogenetic analysis also revealed that majority of the Malaysian commercial chickens were randomly assembled with the Type-II village chickens. In RAPD assay, phylogenetic analysis using neighbor-joining produced six clusters that were completely distinguished based on the locality of chickens. High levels of genetic variations were observed among the village chickens, the commercial broilers, and between the commercial broilers and layer chickens. In this study, it was found that Type-I village chickens could be distinguished from the commercial chickens and Type-II village chickens at the position of the 27th nucleotide of the 351 bp cytochrome b gene. This study also revealed that RAPD markers were unable to differentiate the type of chickens, but it showed the effectiveness of RAPD in evaluating the genetic variation and the genetic relationships between chicken lines and populations.

Item Type: Indexed Article
Subject Heading: Phylogeny
Subject Heading: Broilers (chickens)
Subject Heading: Cladistic analysis
Subject Heading: Animal breeds
Keywords: Cytochrome b gene - Maximum parsimony (MP) - Mitochondrial DNA (mtDNA) - Neighbor-joining (NJ) - Random amplified polymorphic DNA (RAPD)
Faculty: Faculty of Earth Sciences
Deposited By: En. Pahmi Abdullah
Date Deposited: 14 May 2012 20:59
Last Modified: 07 Mar 2016 08:20
URI: http://umkeprints.umk.edu.my/id/eprint/261

Actions (login required)

View Item View Item