Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation

Syarifah Ab Rashid , and Darah Ibrahim, and Ibrahim Che Omar, (2011) Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation. The Internet Journal of Microbiology, 9 (1). pp. 1-19. ISSN 1937-8289

Official URL: http://www.ispub.com/journal/the-internet-journal-...

Abstract

A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30ºC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.

Item Type: Non-Indexed Article
Keywords: Palm Kernel cake, Fermentation
Faculty: Faculty of Agro - Based Industry
Deposited By: En. Pahmi Abdullah
Date Deposited: 13 May 2012 15:39
Last Modified: 04 Feb 2013 10:55
URI: http://umkeprints.umk.edu.my/id/eprint/231

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