Isolation of genomic difference of rubber trees with different Susceptibility to Common Disease in Field.

Liew Kuen Harng, (2012) Isolation of genomic difference of rubber trees with different Susceptibility to Common Disease in Field. Undergraduate project report, Faculty of Agro - Based Industry. (Submitted)

Abstract

in this study, representational difference analysis (RDA) was applied to isolate the genomic difference of rubber trees that is susceptible and resistant to common disease of Colletotrichurn gloeosporioides and Oidium heyecie infection. Samples were collected and categories into six groups based on the severity of disease infection. Samples which are susceptible to disease include group A, B and C. Samples which are tolerance to disease include group D, E and F. DNA extraction was conducted to extract the DNA from the leaves samples with two protocols, which were extraction with liquid nitrogen and extraction with ethanol. Extracted DNA from extraction with ethanol was choosing for further conducted of RDA. RDA was carried out by preparation of amplicons for driver and tester genome. Driver genome consists of the gene pooling of disease susceptible samples and tester genome consists of the gene pooling of disease tolerance samples. After that, kinetic enrichment of DNA through hybridization and amplification was conducted to obtain the genomic difference of resistance gene. For hybridization, tester and driver genome was mixed in the ratio of 1:50 and 1:100 (tester/driver). Distinct fragment of isolate gene was observed in 1.5% agarose gel after the first round of hybridization-amplification process. The result obtained from hybridization with ratio 1-.50 was better than hybridization with ratio 1:100 because hybridization with ratio 1:50 had shorter length of sequence smearing.

Item Type: Undergraduate Final Project Report
Faculty: Faculty of Agro - Based Industry
Call Number: SBT 12007
Supervisor: Miss Raimi Mohamed Redwan
Programme: Bioindustrial Technology
Deposited By: Mohd Suhairi Mohamad
Date Deposited: 17 Nov 2013 13:42
Last Modified: 15 Feb 2017 03:20
URI: http://umkeprints.umk.edu.my/id/eprint/2248

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